We are happy to welcome you to our second Open Educational Resource (OER) textbook, Biochemistry Free For All. Biochemistry is a relatively young science, but its rate of growth has been truly impressive. The rapid pace of discoveries, which shows no sign of slowing, is reflected in the steady increase in the size of biochemistry textbooks. Growing faster than the size of biochemistry books have been the skyrocketing costs of higher education and the even faster rising costs of college textbooks. These unfortunate realities have created a situation where the costs of going to college are beyond the means of increasing numbers of students.

Given the broad definition of biotechnology applications and products, it is easy to see how there is enormous overlap within the fields of cellular biology, microbiology, chemistry, and biomedical engineering. It is the goal of this textbook to provide foundational knowledge to begin building your biotechnology toolkit and enter an exciting career of making a difference through biotechnology.

This textbook first explores the fundamentals of laboratory science and biotechnology. Unit 1 begins with a dive into the foundation of biology and chemistry by asking what is life and what are the molecules of life? The end of the unit offers a primer into laboratory science; preparing solutions and operating basic lab equipment. Unit 2 brings readers through a vital tool of biomanufacturing, understanding the growth and control of microbes. Many biotechnology products are made by harnessing the work of microbes, and this unit explores this invisible and intriguing world of microorganisms. The final unit, molecular biotechnology, offers a more in-depth look at how biological molecules such as DNA and protein are manipulated into creating useful products.

BI102A is a survey course that introduces the discipline of molecular biology and genetics, exploring topics including cell division, protein production, inheritance and gene regulation. This book focuses on putting those topics into an appropriate context for students who are not biology majors. Order a print copy: http://www.lulu.com/content/paperback-book/general-biology-ii-survey-of-molecular-life-and-genetics/23678587

By the end of this section, you will be able to do the following:

List the steps in eukaryotic transcription
Discuss the role of RNA polymerases in transcription
Compare and contrast the three RNA polymerases
Explain the significance of transcription factors

By the end of this section, you will be able to do the following:

List the different steps in prokaryotic transcription
Discuss the role of promoters in prokaryotic transcription
Describe how and when transcription is terminated

By the end of this section, you will be able to do the following:

Describe the different steps in RNA processing
Understand the significance of exons, introns, and splicing for mRNAs
Explain how tRNAs and rRNAs are processed

By the end of this section, you will be able to do the following:

Describe the different steps in protein synthesis
Discuss the role of ribosomes in protein synthesis

By the end of this section, you will be able to do the following:

Explain the “central dogma” of DNA-protein synthesis
Describe the genetic code and how the nucleotide sequence prescribes the amino acid and the protein sequence

By the end of this section, you will be able to do the following:

List the components of the endomembrane system
Recognize the relationship between the endomembrane system and its functions

By the end of this section, you will be able to do the following:

Describe the functions proteins perform in the cell and in tissues
Discuss the relationship between amino acids and proteins
Explain the four levels of protein organization
Describe the ways in which protein shape and function are linked